How to Improve PCR Plate Sealing Success and Reduce Sample Loss in Your Lab

High-throughput labs often face failed sealing of 384-well PCR plates, leading to sample cross-contamination and repeated experiments. This can waste reagents, time, and impact results. Based on feedback from multiple molecular biology labs, here’s a validated approach to improve sealing success and minimize sample loss.

• Mismatched sealing film and well size

• Material elasticity insufficient for high-temperature cycles

• Poor labeling leading to mix-ups during experiments

• Dual-material plates improve elasticity and withstand PCR thermal cycling.

• Pre-labeling batches allows quick comparison and selection.

• Standardized SOP alignment reduces human error.

A U.S. molecular biology lab implemented dual-material PCR plates and reported:

• Sealing failure rate reduced from 8% to 2%

• Experiment cycles shortened by 15%

• Sample loss significantly decreased

Note: These figures are verified from actual lab customer feedback.

Want to test dual-material PCR plates in your lab? Request a sample or contact us for more details.